Polystyrene (PS) and general purpose polystyrene (GPPS) are widely used in medical and biological experimental equipment due to their good chemical stability and biocompatibility. They can resist the erosion of a variety of chemical reagents, maintain the stability of the material, and do not produce adverse reactions with the organism, ensuring the purity and safety of the experimental process.

However, relying solely on the basic properties of the material is not enough to meet the needs of high-precision experiments such as cell culture. In order to ensure that the pipette will not have any adverse effects on cell growth and experimental results during use, the polystyrene or general purpose polystyrene materials used in 10ml cell culture serum pipettes must undergo a series of rigorous screening and testing.

Pyrogen test: Pyrogen is an endogenous pyrogenic substance produced during microbial metabolism, which can cause a fever reaction in organisms. In cell culture experiments, any tiny pyrogen contamination may interfere with the growth state of cells. Therefore, the material of the pipette must undergo pyrogen testing to ensure that pyrogen substances will not be released at high temperatures, thereby ensuring the stability of the experimental environment.

Cytotoxicity test: Cytotoxicity refers to the toxic effect of a substance on cells. For pipettes, the material must be compatible with cells and cannot have any toxic effects on cells. Cytotoxicity testing evaluates the cytotoxicity of materials by simulating the environment in which cells and materials come into contact and observing indicators such as cell growth status and morphological changes. Only materials that have been tested for cytotoxicity and proven to be harmless can be used to make pipettes.

DNA and RNA enzyme tests: In cell culture experiments, the integrity of DNA and RNA is crucial to the accuracy of experimental results. DNA and RNA enzymes are enzymes that can degrade DNA and RNA. Their presence will destroy the nucleic acid structure of the sample and affect the reliability of the experimental results. Therefore, the material of the pipette must be tested for DNA and RNA enzymes to ensure that these enzymes are not contained or released, thereby protecting the nucleic acid integrity of the sample.

Endotoxin test: Endotoxin is a component of the cell wall of Gram-negative bacteria. It has strong pyrogenicity and toxicity. In cell culture experiments, endotoxin contamination may cause cell death, abnormal growth, or deviation in experimental results. Therefore, the material of the pipette must be tested for endotoxins to ensure that there is no endotoxin contamination and to ensure the purity and safety of the experimental process.

These rigorous screening and testing not only ensure the safety and reliability of the pipette material, but also provide strong guarantees for high-precision experiments such as cell culture. Through pyrogen testing, cytotoxicity testing, DNA and RNA enzyme testing, and endotoxin testing, we can ensure that the pipette will not have any adverse effects on cell growth and experimental results during use. This is crucial for scientific researchers who pursue high-precision experimental results.