In the laboratory environment, any minor contamination may have a significant impact on the experimental results. As a bridge connecting samples and test reagents, the sterility of the pipette is the cornerstone of the success of the experiment. The presence of impurities such as dust, fiber, and microorganisms may enter the sample through the pipette, causing cross contamination, which in turn affects the accuracy of the experimental data. Therefore, keeping the operating environment clean and sterile is the primary condition to ensure the quality of the experiment.

As the main place for pipette operation, the cleanliness of the operating table is directly related to the sterility of the pipette. To ensure the cleanliness of the table, users should take the following measures:
Regular cleaning: Use a special laboratory cleaner or 70% ethanol solution to clean the operating table regularly to remove dust, grease and microorganisms attached to the surface. During the cleaning process, ensure that the detergent does not remain to avoid interference with subsequent experiments.
Aseptic operation: Before performing pipetting operations, users should wear sterile gloves and use aseptic techniques to reduce the contamination of hand microorganisms on the operating table and pipette. Avoid placing non-experimental items on the operating table to reduce the source of contamination.
Table material selection: Choose a table material that is easy to clean and corrosion-resistant, such as stainless steel or high-density polyethylene, to reduce the risk of damage and contamination during the cleaning process.

During the operation of the pipette, in addition to keeping the operating table clean, you should also pay attention to the following points to ensure the sterility of the pipette:
Sterile packaging opening: When opening an independently packaged serological pipette, avoid direct contact with the pipette head with your hands. Use sterile tweezers or gloves to operate to reduce the risk of contamination.
Use and storage of pipettes: When using a pipette, follow the manufacturer's instructions to avoid damage or contamination of the pipette due to excessive force or improper operation. After use, the pipette should be returned to a dedicated sterile container to avoid long-term exposure to the air to reduce the chance of contamination.
Regular calibration and disinfection: Regularly calibrate the accuracy of the pipette to ensure its stable performance. At the same time, for frequently used pipettes, regular high-temperature steam sterilization or chemical disinfection should be considered to kill possible microorganisms.

To ensure the continuous cleanliness and sterility of the operating environment, users should also implement environmental monitoring measures to promptly identify and resolve potential problems:

Microbial monitoring: Regularly conduct microbial sampling and testing of the operating environment to understand the microbial contamination in the environment. Once the contamination exceeds the standard, measures should be taken immediately for cleaning and disinfection.

Air cleanliness control: When conditions permit, equipment such as laminar flow hoods or high-efficiency air filters can be used to improve the air cleanliness of the operating environment and reduce the number of microorganisms in the air.

Employee training and awareness enhancement: Regularly conduct aseptic operation training and awareness enhancement for laboratory personnel to ensure that every employee can strictly abide by the aseptic operation procedures and jointly maintain the cleanliness and sterility of the operating environment.